14-3-3 epsilon Antibody (02) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26078
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 02
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 14-3-3 epsilon Antibody (02) - Azide and BSA Free
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon (Accession#: NP_006752.1; Met1-Gln255).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for 14-3-3 epsilon Antibody (02) - Azide and BSA Free
Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] -
Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa
Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] -
Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate 4 uL NBP3-26078 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26078, at 1:100 dilution. Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2 [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDaApplications for 14-3-3 epsilon Antibody (02) - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 14-3-3 epsilon
Alternate Names
1433 epsilon, YWHAE
Gene Symbol
YWHAE
Additional 14-3-3 epsilon Products
Product Documents for 14-3-3 epsilon Antibody (02) - Azide and BSA Free
Product Specific Notices for 14-3-3 epsilon Antibody (02) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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