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14-3-3 epsilon Antibody (4F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59395

Novus Biologicals, part of Bio-Techne
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NBP2-59395-100ul
NBP2-59395-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F8

Format

BSA Free

Concentration

1 mg/ml

Product Summary for 14-3-3 epsilon Antibody (4F8) - BSA Free

Immunogen

Recombinant human 14-3-3 epsilon (1-255aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for 14-3-3 epsilon Antibody (4F8) - BSA Free

Western Blot: 14-3-3 epsilon Antibody (4F8)BSA Free [NBP2-59395]

Western Blot: 14-3-3 epsilon Antibody (4F8)BSA Free [NBP2-59395]

Western Blot: 14-3-3 epsilon Antibody (4F8) [NBP2-59395] - Lane 1: 14-3-3 Zeta Recombinant Protein, Lane 2: 14-3-3 Beta Recombinant Protein, Lane 3: 14-3-3 Epsilon Recombinant Protein, Lane 4: 14-3-3 Eta Recombinant Protein, Lane 5: 14-3-3 Gamma Recombinant Protein, Lane 6: 14-3-3 Sigma Recombinant Protein, Lane 7: 14-3-3 Tau Recombinant Protein
Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody (4F8) - BSA Free [NBP2-59395]

Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody (4F8) - BSA Free [NBP2-59395]

Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody (4F8) [NBP2-59395] - Analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for 14-3-3 epsilon Antibody (4F8) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 epsilon

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody (4F8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 epsilon Antibody (4F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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