14-3-3 epsilon Antibody (CPTC-YWHAE-1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79810
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # CPTC-YWHAE-1
Concentration
0.2 mg/ml
Product Summary for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)
Immunogen
Recombinant human full-length 14-3-3 epsilon protein (Uniprot: P62268)
Localization
Cytoplasm, Melanosome
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79944)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3 epsilon Antibody (CPTC-YWHAE-1).Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810]
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. 14-3-3 epsilon Antibody (NBP2-79810) primary antibody at 1:1000, 4C, overnight. Wb image submitted by a verified customer review.Applications for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)
Application
Recommended Usage
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-79810 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: 14-3-3 epsilon
Additional 14-3-3 epsilon Products
Product Documents for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)
Product Specific Notices for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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