14-3-3 gamma Antibody (13C9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26246
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 13C9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 14-3-3 gamma Antibody (13C9)
Immunogen
A synthesized peptide derived from Human 14-3-3 gamma [UniProt P61981]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 14-3-3 gamma Antibody (13C9)
Immunohistochemistry: 14-3-3 gamma Antibody (13C9) [NBP3-26246] -
Immunohistochemistry: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Image of 14-3-3 gamma Antibody (13C9) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: 14-3-3 gamma Antibody (13C9) [NBP3-26246] -
Western Blot: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, K562 whole cell lysate.All lanes: 14-3-3 gamma Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Applications for 14-3-3 gamma Antibody (13C9)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: 14-3-3 gamma
Signal-induced phosphorylation has the ability to change protein function, however phosphorylation is not always enough to change a protein's function. 14-3-3 gamma has the ability to bind a large number of target proteins thereby affecting the proteins' activity and/or function.
14-3-3 gamma is highly expressed in brain, heart and skeletal muscle, where it is induced by growth factors in human vascular smooth muscle cells. This suggests that 14-3-3 gamma may play a role in muscle tissue function.
Alternate Names
1433 gamma, YWHAG
Gene Symbol
YWHAG
Additional 14-3-3 gamma Products
Product Documents for 14-3-3 gamma Antibody (13C9)
Product Specific Notices for 14-3-3 gamma Antibody (13C9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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