14-3-3 tau/theta Antibody (1A1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21301
![Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]](https://resources.bio-techne.com/images/products/14-3-3-tau-theta-Antibody-1A1-Western-Blot-NBP1-21301-img0005.jpg "Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]")
![Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]](https://resources.bio-techne.com/images/products/14-3-3-tau-theta-Antibody-1A1-Western-Blot-NBP1-21301-img0006.jpg "Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1A1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human 14-3-3 tau/theta (1-245aa) purified from E. coli (NP_006817).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 14-3-3 tau/theta Antibody (1A1) - BSA Free
Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]
Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : MDA-MB-21 cell lysateLane 2. : HeLa cell lysateLane 3. : A431 cell lysateLane 4. : NIH3T3 cell lysateLane 5. : 293T cell lysateLane 6. : HepG2 cell lysate![Immunocytochemistry/ Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) - BSA Free [NBP1-21301]](https://resources.bio-techne.com/images/products/14-3-3-tau-theta-Antibody-1A1-Immunocytochemistry-Immunofluorescence-NBP1-21301-img0003.jpg "Immunocytochemistry/ Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) - BSA Free [NBP1-21301]")
Immunocytochemistry/ Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) - BSA Free [NBP1-21301]
Immunocytochemistry/Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]](https://resources.bio-techne.com/images/products/14-3-3-tau-theta-Antibody-1A1-Western-Blot-NBP1-21301-img0002.jpg "Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]")
Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]
Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - 14-3-3 tau/theta Antibody (1A1) Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 theta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Applications for 14-3-3 tau/theta Antibody (1A1) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000-1:2000
Reviewed Applications
Read 1 review rated 5 using NBP1-21301 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 14-3-3 theta
Alternate Names
14-3-3 protein tau, 1433 theta, HS1 protein, YWHAQ
Entrez Gene IDs
10971 (Human)
Gene Symbol
YWHAQ
UniProt
Additional 14-3-3 theta Products
Product Documents for 14-3-3 tau/theta Antibody (1A1) - BSA Free
Product Specific Notices for 14-3-3 tau/theta Antibody (1A1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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