14-3-3 zeta Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31325
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chicken (99%), Porcine (99%), Rat (99%), Rhesus Macaque (100%), Sheep (99%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (89%).
Localization
Cytoplasm, Melanosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 14-3-3 zeta Antibody - Azide Free
Western Blot: 14-3-3 zeta Antibody [NBP1-31325]
Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325]
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Mouse colon. 14-3-3 zeta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325]
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for 14-3-3 zeta Antibody - Azide Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
Azide Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 14-3-3 zeta
Alternate Names
1433 zeta, KCIP-1, YWHAZ
Gene Symbol
YWHAZ
Additional 14-3-3 zeta Products
Product Documents for 14-3-3 zeta Antibody - Azide Free
Product Specific Notices for 14-3-3 zeta Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.