15-PGDH/HPGD Antibody (027)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89841
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841]](https://resources.bio-techne.com/images/products/15-PGDH-HPGD-Antibody-027-Immunocytochemistry-Immunofluorescence-NBP2-89841-img0001.jpg "Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 027
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human 15-PGDH/HPGD (Accession#: NP_000851.2; Met1-Gln266).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for 15-PGDH/HPGD Antibody (027)
Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841]
Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HPGD monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.![Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841]](https://resources.bio-techne.com/images/products/15-PGDH-HPGD-Antibody-027-Flow-Cytometry-NBP2-89841-img0002.jpg "Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841]")
Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841]
Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated, stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for 15-PGDH/HPGD Antibody (027)
Application
Recommended Usage
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 15-PGDH/HPGD
15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.
Long Name
15-Hydroxyprostaglandin Dehydrogenase
Alternate Names
15PGDH, HPGD, PGDH1, SDR36C1
Gene Symbol
HPGD
Additional 15-PGDH/HPGD Products
Product Documents for 15-PGDH/HPGD Antibody (027)
Product Specific Notices for 15-PGDH/HPGD Antibody (027)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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