2B4/CD244/SLAMF4 Antibody (244F4) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00221
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Flow Cytometry
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 244F4
Concentration
0.5 mg/ml
Product Summary for 2B4/CD244/SLAMF4 Antibody (244F4) [FITC]
Immunogen
The immunogen for this antibody is CD244.
Reactivity Notes
Mouse
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for 2B4/CD244/SLAMF4 Antibody (244F4) [FITC]
Flow Cytometry: 2B4/CD244/SLAMF4 Antibody (244F4) [FITC] [NBP2-00221] - Staining of C57Bl/6 splenocytes with Anti-Mouse NK1.1 PE and 0.125 ug of Rat IgG2a K Isotype Control FITC (left) or 0.125 ug of Anti-Mouse CD244.2 (2B4) FITC (right). Cells in the lymphocyte gate were used for analysis.
Applications for 2B4/CD244/SLAMF4 Antibody (244F4) [FITC]
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Application Notes
This 244F4 antibody has been tested by flow cytometric analysis of IL-2-stimulated mouse splenocytes. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 244F4 antibody has also been reported for use in immunoblotting (WB). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: 2B4/CD244/SLAMF4
The 2B4 receptor has both activating and inhibitory signaling properties (1,4). 2B4's ligand is CD48, which is expressed on hematopoietic cells (4, 5). When bound to CD48, subsequent phosphorylation of the ITSMs occurs, followed by recruitment of SH-2-containing adaptor protein (SAP) and Fyn, leading to phosphorylation of PLCgamma and Vav1, and resulting in NK cell activation (4). In the absence of SAP, Src homology region 2-containing protein tyrosine phosphatases (SHP) get recruited and inhibits NK cell activation (4). In the context of infection such as Epstein-Barr virus (EBV), when lacking SAP, 2B4 has been shown to have an inhibitory function via impaired T cell cytotoxicity against EBV-infected cells (6). While 2B4 has a role in eliciting CD8+ T cell response, overexpression of 2B4 can lead to T cell exhaustion in states of chronic infection (6). Studies have suggested that the antibody blockade of the 2B4-CD48 interaction might enhance T cell and NK cell function, however given both the activating and inhibitory properties of the 2B4 receptor, more research needs to be done to prevent unwanted effects (6).
References
1. Buller CW, Mathew PA, Mathew SO. Roles of NK Cell Receptors 2B4 (CD244), CS1 (CD319), and LLT1 (CLEC2D) in Cancer. Cancers (Basel). 2020 Jul 1;12(7):1755. https://doi.org/10.3390/cancers12071755
2. Catakovic K, Klieser E, Neureiter D, Geisberger R. T cell exhaustion: from pathophysiological basics to tumor immunotherapy. Cell Commun Signal. 2017;15(1):1. https://doi.org/10.1186/s12964-016-0160-z
3. Uniprot (Q9BZW8)
4. Chen Y, Lu D, Churov A, Fu R. Research Progress on NK Cell Receptors and Their Signaling Pathways. Mediators Inflamm. 2020 Jul 24;2020:6437057. https://doi.org/10.1155/2020/6437057.
5. Pahima H, Puzzovio PG, Levi-Schaffer F. 2B4 and CD48: A powerful couple of the immune system. Clin Immunol. 2019 Jul;204:64-68. https://doi.org/10.1016/j.clim.2018.10.014.
6. Waggoner SN, Kumar V. Evolving role of 2B4/CD244 in T and NK cell responses during virus infection. Front Immunol. 2012;3:377. https://doi.org/10.3389/fimmu.2012.00377
Alternate Names
CD244, NAIL, NKR2B4, Nmrk, SLAMF4
Gene Symbol
CD244
Additional 2B4/CD244/SLAMF4 Products
Product Documents for 2B4/CD244/SLAMF4 Antibody (244F4) [FITC]
Product Specific Notices for 2B4/CD244/SLAMF4 Antibody (244F4) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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