2B4/CD244/SLAMF4 Antibody (M053)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23199
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M053
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 2B4/CD244/SLAMF4 Antibody (M053)
Immunogen
Clone (M053) was generated from a recombinant protein that included the extracellular region of human 2B4/CD244/SLAMF4 protein. [UniProt# Q9BZW8]
Specificity
Clone M053 mouse monoclonal antibody detects a 110 kDa protein on SDS-PAGE "Native" or denatured immunoblots of human THP1 monocytes. The antibody prefers the native CD244 protein, but also detects denatured CD244.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for 2B4/CD244/SLAMF4 Antibody (M053)
Western Blot: 2B4/CD244/SLAMF4 Antibody (M053) [NBP3-23199] -
Western Blot: 2B4/CD244/SLAMF4 Antibody (M053) [NBP3-23199] - Western blot of native (lane 1 and 3) and denatured (lane 2 and 4) lysates of human THP-1 monocytes. The blot was probed with mouse monoclonals anti-2B4/CD244/SLAMF4 (NBP3-23199) (lanes 1 and 2) or anti-2B4/CD244/SLAMF4 (NBP3-23211). Both antibodies were used at 1:250.
Immunocytochemistry/Immunofluorescence: 2B4/CD244/SLAMF4 Antibody (M053) [NBP3-23199] -
Immunocytochemistry/Immunofluorescence: 2B4/CD244/SLAMF4 Antibody (M053) [NBP3-23199] - Immunocytochemical labeling of CD244 in aldehyde fixed and NP-40 permeabilized PMA-differentiated human THP-1 cells. The cells were labeled with mouse monoclonal anti-2B4/CD244/SLAMF4 (NBP3-23199). The antibody was detected using goat anti-mouse DyLight(R) 594.
Immunocytochemistry/Immunofluorescence: 2B4/CD244/SLAMF4 Antibody (M053) [NBP3-23199] -
Immunocytochemistry/Immunofluorescence: 2B4/CD244/SLAMF4 Antibody (M053) [NBP3-23199] - Immunocytochemical labeling of CD244 in aldehyde fixed and NP-40 permeabilized PMA-differentiated human THP-1 cells. The cells were labeled with mouse monoclonal anti-2B4/CD244/SLAMF4 (NBP3-23199). The antibody was detected using goat anti-mouse DyLight(R) 594.Applications for 2B4/CD244/SLAMF4 Antibody (M053)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunoprecipitation
1:100
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: 2B4/CD244/SLAMF4
The 2B4 receptor has both activating and inhibitory signaling properties (1,4). 2B4's ligand is CD48, which is expressed on hematopoietic cells (4, 5). When bound to CD48, subsequent phosphorylation of the ITSMs occurs, followed by recruitment of SH-2-containing adaptor protein (SAP) and Fyn, leading to phosphorylation of PLCgamma and Vav1, and resulting in NK cell activation (4). In the absence of SAP, Src homology region 2-containing protein tyrosine phosphatases (SHP) get recruited and inhibits NK cell activation (4). In the context of infection such as Epstein-Barr virus (EBV), when lacking SAP, 2B4 has been shown to have an inhibitory function via impaired T cell cytotoxicity against EBV-infected cells (6). While 2B4 has a role in eliciting CD8+ T cell response, overexpression of 2B4 can lead to T cell exhaustion in states of chronic infection (6). Studies have suggested that the antibody blockade of the 2B4-CD48 interaction might enhance T cell and NK cell function, however given both the activating and inhibitory properties of the 2B4 receptor, more research needs to be done to prevent unwanted effects (6).
References
1. Buller CW, Mathew PA, Mathew SO. Roles of NK Cell Receptors 2B4 (CD244), CS1 (CD319), and LLT1 (CLEC2D) in Cancer. Cancers (Basel). 2020 Jul 1;12(7):1755. https://doi.org/10.3390/cancers12071755
2. Catakovic K, Klieser E, Neureiter D, Geisberger R. T cell exhaustion: from pathophysiological basics to tumor immunotherapy. Cell Commun Signal. 2017;15(1):1. https://doi.org/10.1186/s12964-016-0160-z
3. Uniprot (Q9BZW8)
4. Chen Y, Lu D, Churov A, Fu R. Research Progress on NK Cell Receptors and Their Signaling Pathways. Mediators Inflamm. 2020 Jul 24;2020:6437057. https://doi.org/10.1155/2020/6437057.
5. Pahima H, Puzzovio PG, Levi-Schaffer F. 2B4 and CD48: A powerful couple of the immune system. Clin Immunol. 2019 Jul;204:64-68. https://doi.org/10.1016/j.clim.2018.10.014.
6. Waggoner SN, Kumar V. Evolving role of 2B4/CD244 in T and NK cell responses during virus infection. Front Immunol. 2012;3:377. https://doi.org/10.3389/fimmu.2012.00377
Alternate Names
CD244, NAIL, NKR2B4, Nmrk, SLAMF4
Gene Symbol
CD244
Additional 2B4/CD244/SLAMF4 Products
Product Documents for 2B4/CD244/SLAMF4 Antibody (M053)
Product Specific Notices for 2B4/CD244/SLAMF4 Antibody (M053)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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