2B4/CD244/SLAMF4 [p Tyr271] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46104

Novus Biologicals, part of Bio-Techne
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NBP1-46104
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 2B4/CD244/SLAMF4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EFLTIpYEDVKD corresponding to internal region according to NP_057466.1.

Epitope

internal region

Modification

p Tyr271

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 2B4/CD244/SLAMF4 [p Tyr271] Antibody

Western Blot: 2B4/CD244/SLAMF4 [p Tyr271] Antibody [NBP1-46104]

Western Blot: 2B4/CD244/SLAMF4 [p Tyr271] Antibody [NBP1-46104]

Western Blot: 2B4/CD244/SLAMF4 [p Tyr271] Antibody [NBP1-46104] - Analysis of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells using this antibody at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas, Sir William Dunn.

Applications for 2B4/CD244/SLAMF4 [p Tyr271] Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human Natural Killer (NK) lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1 kDa band according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 2B4/CD244/SLAMF4

2B4, also known as CD244 and SLAMF4, is a type I transmembrane glycoprotein that is member of the signaling lymphocyte activation molecule (SLAM) subfamily in immunoglobulin (Ig) superfamily (1,2). The 2B4 protein plays a role in immune system associated diseases including the recognition and clearance of cancer cells, infected cells, and key allergy cells (1). 2B4 is expressed as a heterophilic receptor on the cell membrane of natural killer (NK) cells in addition to on monocytes and basophils, gamma/delta T cells, a subset of CD8+ T cells, and other immune cells and tissues (1). Additionally, 2B4 is considered a marker for CD8+ T cell exhaustion in chronic infection and increased expression has been observed in diseases including lymphocytic choriomeningitis virus (LCMV) and hepatitis B virus (HBV) (2). Human 2B4 protein is 370 amino acids (aa) in length with a theoretical molecular weight (MW) of 41 kDa (3). However, 2B4 contains multiple glycosylation sites that increases its observed MW to ~70 kDa (3). The 2B4/CD244 protein consists of a 21 aa signal peptide followed by an extracellular domain (ECD) with two Ig-like domains, a single transmembrane domain, and four cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs) (3).

The 2B4 receptor has both activating and inhibitory signaling properties (1,4). 2B4's ligand is CD48, which is expressed on hematopoietic cells (4, 5). When bound to CD48, subsequent phosphorylation of the ITSMs occurs, followed by recruitment of SH-2-containing adaptor protein (SAP) and Fyn, leading to phosphorylation of PLCgamma and Vav1, and resulting in NK cell activation (4). In the absence of SAP, Src homology region 2-containing protein tyrosine phosphatases (SHP) get recruited and inhibits NK cell activation (4). In the context of infection such as Epstein-Barr virus (EBV), when lacking SAP, 2B4 has been shown to have an inhibitory function via impaired T cell cytotoxicity against EBV-infected cells (6). While 2B4 has a role in eliciting CD8+ T cell response, overexpression of 2B4 can lead to T cell exhaustion in states of chronic infection (6). Studies have suggested that the antibody blockade of the 2B4-CD48 interaction might enhance T cell and NK cell function, however given both the activating and inhibitory properties of the 2B4 receptor, more research needs to be done to prevent unwanted effects (6).

References

1. Buller CW, Mathew PA, Mathew SO. Roles of NK Cell Receptors 2B4 (CD244), CS1 (CD319), and LLT1 (CLEC2D) in Cancer. Cancers (Basel). 2020 Jul 1;12(7):1755. https://doi.org/10.3390/cancers12071755

2. Catakovic K, Klieser E, Neureiter D, Geisberger R. T cell exhaustion: from pathophysiological basics to tumor immunotherapy. Cell Commun Signal. 2017;15(1):1. https://doi.org/10.1186/s12964-016-0160-z

3. Uniprot (Q9BZW8)

4. Chen Y, Lu D, Churov A, Fu R. Research Progress on NK Cell Receptors and Their Signaling Pathways. Mediators Inflamm. 2020 Jul 24;2020:6437057. https://doi.org/10.1155/2020/6437057.

5. Pahima H, Puzzovio PG, Levi-Schaffer F. 2B4 and CD48: A powerful couple of the immune system. Clin Immunol. 2019 Jul;204:64-68. https://doi.org/10.1016/j.clim.2018.10.014.

6. Waggoner SN, Kumar V. Evolving role of 2B4/CD244 in T and NK cell responses during virus infection. Front Immunol. 2012;3:377. https://doi.org/10.3389/fimmu.2012.00377

Alternate Names

CD244, NAIL, NKR2B4, Nmrk, SLAMF4

Entrez Gene IDs

51744 (Human)

Gene Symbol

CD244

UniProt

NP_057466.1 (Human)

Additional 2B4/CD244/SLAMF4 Products

Product Documents for 2B4/CD244/SLAMF4 [p Tyr271] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 2B4/CD244/SLAMF4 [p Tyr271] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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