4-1BB Ligand/TNFSF9 Antibody (014)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90388

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 014

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 4-1BB Ligand/TNFSF9 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse 4-1BB Ligand/TNFSF9 (Arg 104-Glu 309; Accession#: NP_033430.1).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 4-1BB Ligand/TNFSF9 Antibody (014)

Immunocytochemistry/ Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388]

Immunocytochemistry/ Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388]

Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow Cytometry: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388]

Flow Cytometry: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388]

Flow Cytometry: 4-1BB Ligand/TNFSF9 Antibody (014) [NBP2-90388] - Analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for 4-1BB Ligand/TNFSF9 Antibody (014)

Application
Recommended Usage

ELISA

1:1000-1:2000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 4-1BB Ligand/TNFSF9

CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60% amino acid identity. CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen over stimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Gene Symbol

TNFSF9

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for 4-1BB Ligand/TNFSF9 Antibody (014)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 4-1BB Ligand/TNFSF9 Antibody (014)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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