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4-1BB Ligand/TNFSF9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20673

Novus Biologicals, part of Bio-Techne
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NBP2-20673

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human 4-1BB Ligand/TNFSF9. The exact sequence is proprietary.

Localization

Membrane; Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 4-1BB Ligand/TNFSF9 Antibody

Western Blot: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673]

Western Blot: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673]

Western Blot: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.
Immunoprecipitation: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673]

Immunoprecipitation: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673]

Immunoprecipitation: 4-1BB Ligand/TNFSF9 Antibody [NBP2-20673] - IP samples: A549 membrane extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of TNFSF9 protein by 4 ug TNFSF9 antibody [N2C3] 15 % SDS-PAGE The immunoprecipitated TNFSF9 protein was detected by TNFSF9 antibody [N2C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for 4-1BB Ligand/TNFSF9 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 4-1BB Ligand/TNFSF9

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Gene Symbol

TNFSF9

UniProt

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for 4-1BB Ligand/TNFSF9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 4-1BB Ligand/TNFSF9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.