Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 12F7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 4-Hydroxynonenal Antibodies »

Product Specifications

Immunogen

Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH)

Specificity

Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for 4-Hydroxynonenal Antibody (12F7)

Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353]

Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353]

Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (NBP2-59353) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353]

Immunocytochemistry/ Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353]

Immunocytochemistry/Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (NBP2-59353) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunocytochemistry/ Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353]

Immunocytochemistry/ Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353]

Immunocytochemistry/Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.

Applications for 4-Hydroxynonenal Antibody (12F7)

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: 4-Hydroxynonenal

4-HNE is a hydroxyalkenal which is produced by lipid peroxidation in cells. It is found in higher quantities during stress, due to the increase in the lipid peroxidation reaction that accompanies stress events. 4-Hydroxynonenanal is believed to play a key role in signal transduction in a number of pathways, including cellular adhesion.

Alternate Names

4Hydroxynonenal, HNE

Additional 4-Hydroxynonenal Products

Product Documents for 4-Hydroxynonenal Antibody (12F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 4-Hydroxynonenal Antibody (12F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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