4EBP1 Antibody (007) [Janelia Fluor® 669]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89207JF669

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NBP2-89207

Alexa Fluor 350

NBP2-89207AF350

Alexa Fluor 405

NBP2-89207AF405

Alexa Fluor 488

NBP2-89207AF488

Alexa Fluor 532

NBP2-89207AF532

Alexa Fluor 594

NBP2-89207AF594

Alexa Fluor 647

NBP2-89207AF647

Alexa Fluor 700

NBP2-89207AF700

Alexa Fluor 750

NBP2-89207AF750

Allophycocyanin

NBP2-89207APC

Biotin

NBP2-89207B

CoraFluor 1

NBP2-89207CL1

DyLight 350

NBP2-89207UV

DyLight 405

NBP2-89207V

DyLight 488

NBP2-89207G

DyLight 550

NBP2-89207R

DyLight 594

NBP2-89207DL594

DyLight 650

NBP2-89207C

DyLight 680

NBP2-89207FR

DyLight 755

NBP2-89207IR

FITC

NBP2-89207F

HRP

NBP2-89207H

Janelia Fluor 525

NBP2-89207JF525

Janelia Fluor 549

NBP2-89207JF549

Janelia Fluor 585

NBP2-89207JF585

Janelia Fluor 635

NBP2-89207JF635

Janelia Fluor 646

NBP2-89207JF646

mFluor Violet 450 SE

NBP2-89207MFV450

mFluor Violet 500 SE

NBP2-89207MFV500

mFluor Violet 610 SE

NBP2-89207MFV610

PE

NBP2-89207PE

PerCP

NBP2-89207PCP

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Janelia Fluor 669

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 4EBP1 Antibodies »

Product Summary for 4EBP1 Antibody (007) [Janelia Fluor® 669]

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human 4EBP1 (Accession#: NP_004086.1; Ser2-Ile118).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for 4EBP1 Antibody (007) [Janelia Fluor® 669]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 4EBP1

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Gene Symbol

EIF4EBP1

Additional 4EBP1 Products

Product Documents for 4EBP1 Antibody (007) [Janelia Fluor® 669]

Product Datasheets

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COA

SDS

Product Specific Notices for 4EBP1 Antibody (007) [Janelia Fluor® 669]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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