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5-HT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56352

Novus Biologicals, part of Bio-Techne
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NB100-56352SS
NB100-56352

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).

Reactivity Notes

This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with canine 5-HT7Ra/b, and 81% conserved with porcine 5-HT7R.

Specificity

The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NB100-56352]

Western Blot: 5-HT7 Antibody [NB100-56352]

Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.
Simple Western: 5-HT7 Antibody [NB100-56352]

Simple Western: 5-HT7 Antibody [NB100-56352]

Simple Western: 5-HT7 Antibody [NB100-56352] - Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student's t-test. Image collected and cropped by CiteAb from the following publication (h//pubmed.ncbi.nlm.nih.gov/33572981/) licensed under a CC-BY license.
5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NB100-56352] -

Western Blot: 5-HT7 Antibody [NB100-56352] - Effects of subchronic administration (7 and 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) and interaction between Brex and 5-HT1A receptor (5-HT1AR) antagonist WAY100635 (WAY: 10 uM) on protein expression of 5-HT7 receptor in the plasma membrane fraction of cortical primary cultured astrocytes. In left side histograms, ordinate: mean +/- SD (n = 6) of the relative protein level of 5-HT7R per GAPDH. * p < 0.05, ** p < 0.01: relative to control (Brex-free) by one-way analysis of variance (ANOVA) with Tukey’s post-hoc test, and @ p < 0.05: relative to Brex for 14 days by Student’s T-test. Right side panels indicate their pseudo-gel images using capillary immunoblotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35743014/) licensed under a CC-BY license.

Applications for 5-HT7 Antibody

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 30602786)

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (PMID 17940054)

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT7

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs.

Long Name

5-Hydroxytryptamine Receptor 7

Alternate Names

5-HT-X, 5HT7, HTR7

Entrez Gene IDs

3363 (Human); 15566 (Mouse); 65032 (Rat)

Gene Symbol

HTR7

UniProt

Additional 5-HT7 Products

Product Documents for 5-HT7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-HT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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