5-HT7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56352
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine
Cited:
Human, Mouse, Rat
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 13-28 of the rat 5-HT7R (AAA42134.1).
Reactivity Notes
This sequence is identical for 5-HT7R splice variants in the rat (5-HT7Ra/b/c), human (5-HT7Ra/b/d) and human 5-HT7, the mouse 5-HT7R. It is 93% conserved with canine 5-HT7Ra/b, and 81% conserved with porcine 5-HT7R.
Specificity
The 5HT7 antibody recognizes all described 5HT7 receptor splice variants.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 5-HT7 Antibody
Western Blot: 5-HT7 Antibody [NB100-56352]
Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.Simple Western: 5-HT7 Antibody [NB100-56352]
Simple Western: 5-HT7 Antibody [NB100-56352] - Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student's t-test. Image collected and cropped by CiteAb from the following publication (h//pubmed.ncbi.nlm.nih.gov/33572981/) licensed under a CC-BY license.Western Blot: 5-HT7 Antibody [NB100-56352] -
Western Blot: 5-HT7 Antibody [NB100-56352] - Effects of subchronic administration (7 and 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) and interaction between Brex and 5-HT1A receptor (5-HT1AR) antagonist WAY100635 (WAY: 10 uM) on protein expression of 5-HT7 receptor in the plasma membrane fraction of cortical primary cultured astrocytes. In left side histograms, ordinate: mean +/- SD (n = 6) of the relative protein level of 5-HT7R per GAPDH. * p < 0.05, ** p < 0.01: relative to control (Brex-free) by one-way analysis of variance (ANOVA) with Tukey’s post-hoc test, and @ p < 0.05: relative to Brex for 14 days by Student’s T-test. Right side panels indicate their pseudo-gel images using capillary immunoblotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35743014/) licensed under a CC-BY license.Applications for 5-HT7 Antibody
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 30602786)
Immunocytochemistry/ Immunofluorescence
1:10-1:2000. Use reported in scientific literature (PMID 17940054)
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 5-HT7
Long Name
5-Hydroxytryptamine Receptor 7
Alternate Names
5-HT-X, 5HT7, HTR7
Gene Symbol
HTR7
UniProt
Additional 5-HT7 Products
Product Documents for 5-HT7 Antibody
Product Specific Notices for 5-HT7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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