5-HT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46598

Novus Biologicals, part of Bio-Techne
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NBP1-46598
NBP1-46598-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all 5-HT7 Antibodies »

Product Specifications

Immunogen

A synthetic peptide from human 5-HT7 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and guinea pig.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-HT7 Antibody

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598]

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598]

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 Aum.
Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598]

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598]

Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.

Applications for 5-HT7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Frozen sections. Use in ICC/IF reported in scientific literature (PMID 28690143).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT7

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs (

Long Name

5-Hydroxytryptamine Receptor 7

Alternate Names

5-HT-X, 5HT7, HTR7

Entrez Gene IDs

3363 (Human); 15566 (Mouse); 65032 (Rat); 100135549 (Guinea Pig)

Gene Symbol

HTR7

UniProt

P34969

Additional 5-HT7 Products

Product Documents for 5-HT7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 5-HT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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