5-Lipoxygenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53782

Novus Biologicals, part of Bio-Techne
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NB100-53782
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 5-Lipoxygenase Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ERNKKKQLPYYYLSPD corresponding to C-Terminus according to NP_000689.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-Lipoxygenase Antibody

Western Blot: 5-Lipoxygenase Antibody [NB100-53782]

Western Blot: 5-Lipoxygenase Antibody [NB100-53782]

Western Blot: 5-Lipoxygenase Antibody [NB100-53782] - (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for 5-Lipoxygenase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 85 kDa band observed in human bone marrow and rat stomach lysates (calculated MW of 77.7.0 kDa band according to rat NP_036954).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-Lipoxygenase

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Entrez Gene IDs

240 (Human); 11689 (Mouse); 25290 (Rat)

Gene Symbol

ALOX5

UniProt

P09917 (Human)

Additional 5-Lipoxygenase Products

Product Documents for 5-Lipoxygenase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 5-Lipoxygenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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