5-Lipoxygenase [p Ser523] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-277

Novus Biologicals, part of Bio-Techne
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NB300-277
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 5-Lipoxygenase Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH. Accession # P09917

Modification

p Ser523

Localization

Cytoplasmic

Specificity

Specific for endogenous levels of the ~80 kD 5-Lipoxygenase phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-phosphatase, 1200 units for 30 min).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-Lipoxygenase [p Ser523] Antibody

Western Blot: 5-Lipoxygenase [p Ser523] Antibody [NB300-277]

Western Blot: 5-Lipoxygenase [p Ser523] Antibody [NB300-277]

Western Blot: 5-Lipoxygenase [p Ser523] Antibody [NB300-277] - Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for 5-Lipoxygenase [p Ser523] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-Lipoxygenase

The enzyme 5-lipoxygenase (5-LO) play a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contribute to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994) fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis, and atherosclerosis.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Entrez Gene IDs

240 (Human); 25290 (Rat)

Gene Symbol

ALOX5

UniProt

P09917 (Human)

Additional 5-Lipoxygenase Products

Product Documents for 5-Lipoxygenase [p Ser523] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 5-Lipoxygenase [p Ser523] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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