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5-Lipoxygenase [p Ser271] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05439

Novus Biologicals, part of Bio-Techne
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NBP3-05439

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Immunogen

A synthetic phosphorylated peptide around S271 of human 5-Lipoxygenase (NP_000689.1). QLSLE

Modification

p Ser271

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Western Blot: 5-Lipoxygenase [p Ser271] AntibodyBSA Free [NBP3-05439]

Western Blot: 5-Lipoxygenase [p Ser271] AntibodyBSA Free [NBP3-05439]

Western Blot: 5-Lipoxygenase [p Ser271] Antibody [NBP3-05439] - Western blot analysis of extracts of HT-29 cells, using 5-Lipoxygenase antibody (NBP3-05439) at 1:1000 dilution. HT-29 cells were treated by PMA/TPA (200nM). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Applications for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-Lipoxygenase

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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