53BP1 Antibody (6B3E10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80549

Novus Biologicals, part of Bio-Techne
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NBP2-80549
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Catalog #
BSA Free
NBP2-25028
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Canine (93%), Chimpanzee (99%), Equine (91%), Feline (91%), Orangutan (99%), Porcine (91%), Primate (98%), Rabbit (92%). Backed by our 100% Guarantee.

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6B3E10

Format

Azide and BSA Free

Concentration

1 mg/ml

View all 53BP1 Antibodies »

Product Specifications

Immunogen

53BP1 Antibody (6B3E10) was made to a partial recombinant human 53BP1 (between residues 500-800) expressed in E. coli

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (99%), Orangutan (99%), Gibbon (98%), Gorilla (98%), Marmoset (95%), Canine (93%), Rabbit (92%), Feline (91%), Equine (91%), Panda (91%), Porcine (91%)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

213 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 53BP1 Antibody (6B3E10) - Azide and BSA Free

Western Blot: 53BP1 Antibody (6B3E10)Azide and BSA Free [NBP2-80549]

Western Blot: 53BP1 Antibody (6B3E10)Azide and BSA Free [NBP2-80549]

Western Blot: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549] - Western blot analysis using against HEK293 (1) and 53BP1 (AA: 574-773)-hIgGFc transfected HEK293 (2) cell lysate. The observed molecular weight is ~52 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549]

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody [6B3E10] NBP2-80549 at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Beta tubulin NB600-936 was used as a co-stain at a 1:1000 dilution and detected with an anti-rabbit DyLight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549]

Immunohistochemistry-Paraffin: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549]

Immunohistochemistry-Paraffin: 53BP1 Antibody (6B3E10) - Azide and BSA Free [NBP2-80549] - Analysis of paraffin-embedded endometrial cancer tissues using 53BP1 Antibody with DAB staining. Image from the standard format of this antibody.
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Applications for 53BP1 Antibody (6B3E10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

reported by customer review

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 53BP1

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Long Name

p53 Binding Protein 1

Alternate Names

p202, TP53BP1

Gene Symbol

TP53BP1

Additional 53BP1 Products

Product Documents for 53BP1 Antibody (6B3E10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 53BP1 Antibody (6B3E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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