53BP1 Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NB100-926
Key Product Details
Species
Human, Mouse, Rat, Bat, Bovine, Canine, Fish, Goat, Naked mole-rat, Primate
Applications
Chromatin Immunoprecipitation, Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Knockout Validated, Western Blot
Product Summary for 53BP1 Antibody Pack
This pack contains 1 vial each of: NB100-304 (0.1 mL) and NB100-305 (0.1 mL).
Product Specifications
Specificity
The antibodies included in 53BP1 Antibody Pack are specific for 53BP1.
Application Notes
Antibodies in this pack are validated for the following applications:
NB100-305: Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
NB100-304: Chromatin Immunoprecipitation, Flow (Intracellular), Flow Cytometry, Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In-situ Hybridization, Knockdown Validated, Knockout Validated, Western Blot
NB100-305: Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
NB100-304: Chromatin Immunoprecipitation, Flow (Intracellular), Flow Cytometry, Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In-situ Hybridization, Knockdown Validated, Knockout Validated, Western Blot
Reactivity Notes
Antibodies in this pack are validated for use in the following species:
NB100-305: Bat, Bovine, Canine, Human, Mouse, Naked mole-rat, Rat
NB100-304: Bat, Bovine, Canine, Fish, Goat, Human, Mouse, Primate, Rat
Predicted cross-reactivity based on sequence identity: Chimpanzee (99%), Orangutan (99%), Gorilla (98%), Gibbon (98%), Marmoset (95%)
NB100-305: Bat, Bovine, Canine, Human, Mouse, Naked mole-rat, Rat
NB100-304: Bat, Bovine, Canine, Fish, Goat, Human, Mouse, Primate, Rat
Predicted cross-reactivity based on sequence identity: Chimpanzee (99%), Orangutan (99%), Gorilla (98%), Gibbon (98%), Marmoset (95%)
Scientific Data Images for 53BP1 Antibody Pack
Knockout Validated: 53BP1 Antibody Pack [NB100-926]
Knockout Validated: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.Western Blot: 53BP1 Antibody Pack [NB100-926]
Western Blot: 53BP1 Antibody Pack [NB100-926] - Western blot analysis of human 53BP1, blotted with 53BP1 antibody (Catalog #NB100-305). Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926]
Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue) using NB100-305. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY license.Kit Contents for 53BP1 Antibody Pack
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative
0.09% Sodium Azide
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C.
Background: 53BP1
References
1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936
2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749
Long Name
p53 Binding Protein 1
Alternate Names
p202, TP53BP1
Gene Symbol
TP53BP1
Additional 53BP1 Products
Product Documents for 53BP1 Antibody Pack
Product Specific Notices for 53BP1 Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...