58K Golgi Protein Antibody (58K-9)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-412

Novus Biologicals, part of Bio-Techne
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NB600-412
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken

Cited:

Human, Mouse, Rat, Avian - Chicken

Predicted:

Chinese Hamster (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 58K-9

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all 58K Golgi Protein Antibodies »

Product Specifications

Immunogen

58K Golgi Protein purified from rat liver [UniProt# O88618]

Localization

Cytoplasmic and Golgi Apparatus

Marker

Golgi Apparatus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 58K Golgi Protein Antibody (58K-9)

Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412]

Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412]

Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412]

Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412]

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were then incubated with 1:200 dilution of anti-58K Golgi Protein antibody (clone 58K-9) for overnight at 4C and detected with an anti-mouse Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) [NBP2-31156]. Cells were imaged using a 40X objective. Antibody clone 58K-9 generated a specific signal in the Golgi apparatus of the cells.
Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412]

Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412]

Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc and 58K Golgi. Bar is 50 um. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088226) licensed under a CC-BY license.
View 3 more images

Applications for 58K Golgi Protein Antibody (58K-9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:250

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24505439)

Immunohistochemistry-Paraffin

1:100 - 1:200

Western Blot

1:2000 - 1:5000
Application Notes
In Western blot a band is observed at ~58 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.3 using NB600-412 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 58K Golgi Protein

The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).

Alternate Names

formimidoyltransferase cyclodeaminase, formimidoyltransferase-cyclodeaminase, formiminotransferase cyclodeaminase, Formiminotransferase-cyclodeaminase, human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase, LCHC1

Entrez Gene IDs

10841 (Human); 14317 (Mouse); 89833 (Rat)

Gene Symbol

FTCD

UniProt

O95954 (Human), Q91XD4 (Mouse), O88618 (Rat)

Additional 58K Golgi Protein Products

Product Documents for 58K Golgi Protein Antibody (58K-9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 58K Golgi Protein Antibody (58K-9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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