5T4 Antibody (6E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26276
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6E9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 5T4 Antibody (6E9)
Immunogen
A synthesized peptide derived from Human 5T4 [UniProt Q13641]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 5T4 Antibody (6E9)
Immunohistochemistry: 5T4 Antibody (6E9) [NBP3-26276] -
Immunohistochemistry: 5T4 Antibody (6E9) [NBP3-26276] - Image of 5T4 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: 5T4 Antibody (6E9) [NBP3-26276] -
Western Blot: 5T4 Antibody (6E9) [NBP3-26276] - Positive Western Blot detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate.All lanes: 5T4 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 47 kDa
Observed band size: 50, 80 kDa
Applications for 5T4 Antibody (6E9)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: 5T4
Long Name
Oncofetal Antigen/Trophoblast Glycoprotein
Alternate Names
M6P1, TPBG
Gene Symbol
TPBG
Additional 5T4 Products
Product Documents for 5T4 Antibody (6E9)
Product Specific Notices for 5T4 Antibody (6E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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