6 Phosphofructo 2 Kinase Antibody (3F3)
Novus Biologicals, part of Bio-Techne | Catalog # H00005209-M08
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3F3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PFKFB3 (NP_004557, 412 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 22421967). Mouse reactivity reported in scientific literature (PMID: 24169697).
Specificity
PFKFB3 - 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (3F3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for 6 Phosphofructo 2 Kinase Antibody (3F3)
![Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]](https://resources.bio-techne.com/images/products/6-Phosphofructo-2-Kinase-Antibody-3F3-Western-Blot-H00005209-M08-img0005.jpg "Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]")
Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]
Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Analysis of PFKFB3 expression in transfected 293T cell line by PFKFB3 monoclonal antibody (M08), clone 3F3. Lane 1: PFKFB3 transfected lysatE (59.6 KDa). Lane 2: Non-transfected lysate.![Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]](https://resources.bio-techne.com/images/products/6-Phosphofructo-2-Kinase-Antibody-3F3-Western-Blot-H00005209-M08-img0004.jpg "Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]")
Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]
Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Analysis of PFKFB3 transfected lysate using anti-PFKFB3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PFKFB3 rabbit polyclonal antibody.
ELISA: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Detection limit for recombinant GST tagged PFKFB3 is approximately 3ng/ml as a capture antibody.
Applications for 6 Phosphofructo 2 Kinase Antibody (3F3)
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 review rated 4 using H00005209-M08 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PFKFB3
Long Name
6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase
Alternate Names
6 Phosphofructo 2 Kinase, IPFK2, PFK/FBPase 3, PFK2
Entrez Gene IDs
5209 (Human)
Gene Symbol
PFKFB3
UniProt
Additional PFKFB3 Products
Product Documents for 6 Phosphofructo 2 Kinase Antibody (3F3)
Product Specific Notices for 6 Phosphofructo 2 Kinase Antibody (3F3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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