A-RAF Antibody (4U9L7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16677

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16677-100ul
NBP3-16677-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4U9L7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all A-Raf Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 166-290 of human A-RAF (P10398). RQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for A-RAF Antibody (4U9L7)

Western Blot: A-RAF Antibody (4U9L7) [NBP3-16677]

Western Blot: A-RAF Antibody (4U9L7) [NBP3-16677]

Western Blot: A-RAF Antibody (4U9L7) [NBP3-16677] - Western blot analysis of extracts of various cell lines, using A-RAF antibody (NBP3-16677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: A-RAF Antibody (4U9L7) [NBP3-16677]

Immunohistochemistry-Paraffin: A-RAF Antibody (4U9L7) [NBP3-16677]

Immunohistochemistry-Paraffin: A-RAF Antibody (4U9L7) [NBP3-16677] - Immunohistochemistry of paraffin-embedded human liver using A-RAF Rabbit mAb (NBP3-16677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for A-RAF Antibody (4U9L7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:10-1:20

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: A-Raf

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Long Name

v-Raf Murine Sarcoma 3611 Viral Oncogene Homolog

Alternate Names

ARaf, PKS, PKS2, RAFA1

Gene Symbol

ARAF

Additional A-Raf Products

Product Documents for A-RAF Antibody (4U9L7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for A-RAF Antibody (4U9L7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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