A20/TNFAIP3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77351
![Western Blot: A20/TNFAIP3 AntibodyBSA Free [NBP1-77351]](https://resources.bio-techne.com/images/products/A20-TNFAIP3-Antibody-Western-Blot-NBP1-77351-img0001.jpg "Western Blot: A20/TNFAIP3 AntibodyBSA Free [NBP1-77351]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human TNFAIP3. The immunogen is located within the last 50 amino acids of A20/TNFAIP3 Antibody. Amino Acid Squence: HRGEPAPEDPPKQR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for A20/TNFAIP3 Antibody - BSA Free
Western Blot: A20/TNFAIP3 AntibodyBSA Free [NBP1-77351]
Western Blot: A20/TNFAIP3 Antibody [NBP1-77351] - SK-N-SH cell lysate with TNFAIP3 antibody at 1 ug/mL![Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351]](https://resources.bio-techne.com/images/products/A20-TNFAIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77351-img0003.jpg "Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351]")
Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351]
Immunocytochemistry/Immunofluorescence: A20/TNFAIP3 Antibody [NBP1-77351] - Immunofluorescence of A20/TNFAIP3 in Human Lung cells with A20/TNFAIP3 antibody at 20 ug/mL.![Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351]](https://resources.bio-techne.com/images/products/A20-TNFAIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-77351-img0002.jpg "Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351]")
Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody - BSA Free [NBP1-77351]
Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody [NBP1-77351] - Human lung tissue with TNFAIP3 antibody at 5 ug/ml.Applications for A20/TNFAIP3 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunofluorescence
20 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: A20/TNFAIP3
A20/TNFAIP3 is a highly conserved protein sharing >90% amino acid sequence identity across various mammalian species and is highly expressed in T-cell and B-cells. Coding and non-coding single nucleotide polymorphisms (SNPs) of A20/TNFAIP3 have been associated with multiple autoinflammatory and autoimmune diseases including type 1 diabetes, rheumatoid arthritis, Crohn's disease, and systemic lupus erythematosus (SLE) (3). The two domains of A20/TNFAIP3 cooperate to regulate NF-kB signaling. Its N-terminal ovarian tumor (OTU) domain contains a catalytic cysteine (C103) which functions as a K63 deubiquitinase, whereas the 7 zinc fingers that make up the C-terminal domain mediate K48 polyubiquitination. To regulate NF-kB signaling, A20/TNFAIP3 removes K63-polyubiquitin chains from receptor-interacting protein 1 (RIP1) and NF-kB essential modulator (NEMO), thus preventing interactions with downstream partners. A20/TNFAIP3 also contributes to the degradation of RIP1 and Ubc13 through the addition of K48 polyubiquitin chains (4).
References
1.Dixit VM1, Green S, Sarma V, Holzman LB, Wolf FW, O'Rourke K, Ward PA, Prochownik EV, Marks RM. (1990) Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin. J Biol Chem. 265(5):2973-8. PMID: 2406243
2.Verstrepen L, Verhelst K, van Loo G, Carpentier I, Ley SC, Beyaert R. (2010) Expression, biological activities and mechanisms of action of A20 (TNFAIP3). Biochem Pharmacol. 80(12):2009-20. PMID: 20599425
3.Mele A, Cervantes JR, Chien V, Friedman D, Ferran C. (2014) Single nucleotide polymorphisms at the TNFAIP3/A20 locus and susceptibility/resistance to inflammatory and autoimmune diseases. Adv Exp Med Biol. 809:163-83. PMID: 25302371
4.Das T, Chen Z, Hendriks RW, Kool M. (2018) A20/Tumor Necrosis Factor alpha-Induced Protein 3 in Immune Cells Controls Development of Autoinflammation and Autoimmunity: Lessons from Mouse Models. Front Immunol. 9:104. PMID: 29515565
Additional A20/TNFAIP3 Products
Product Documents for A20/TNFAIP3 Antibody - BSA Free
Product Specific Notices for A20/TNFAIP3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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