A2BP1 Antibody (D8H8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13169
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Frozen, PCR
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # D8H8
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]
Localization
Nucleus. Cytoplasm. Note: Predominantly nuclear.
Specificity
Does not cross react with paralogue FOX2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for A2BP1 Antibody (D8H8)
Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169]
Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169]
Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169]
Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.Applications for A2BP1 Antibody (D8H8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:200
Immunohistochemistry-Frozen
reported in scientific literature (PMID 30001398)
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:10-1:100
Simple Western
1:20
Western Blot
1:1000
Application Notes
In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine (pH 7.5) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: A2BP1
Alternate Names
2BP1, A2BP, A2BP1 Hexaribonucleotide-binding protein 1, Ataxin-2-binding protein 1, FOX-1, FOX1 hexaribonucleotide binding protein 1, Fox-1 homolog A, HRNBP1 ataxin 2-binding protein 1, RNA binding protein fox-1 homolog 1, RNA binding protein, fox-1 homolog (C. elegans) 1
Gene Symbol
RBFOX1
UniProt
Additional A2BP1 Products
Product Documents for A2BP1 Antibody (D8H8)
Product Specific Notices for A2BP1 Antibody (D8H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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