A33/GPA33 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89332

Novus Biologicals, part of Bio-Techne
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NBP1-89332
NBP1-89332-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all A33 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TYECSVSLMSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQSKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTSSNEEGTQFCNITVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for A33/GPA33 Antibody

Western Blot: A33/GPA33 Antibody [NBP1-89332]

Western Blot: A33/GPA33 Antibody [NBP1-89332]

Western Blot: A33/GPA33 Antibody [NBP1-89332] - Analysis in human colon tissue.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human colon shows high expression.
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Applications for A33/GPA33 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: A33

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Gene Symbol

GPA33

Additional A33 Products

Product Documents for A33/GPA33 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for A33/GPA33 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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