ABCF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18889

Novus Biologicals, part of Bio-Techne
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NBP1-18889
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ABCF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 795 and 845 of human ATP-binding cassette 50 using the numbering given in entry NP_001020262.1 (GeneID 23).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABCF1 Antibody

Western Blot: ABCF1 Antibody [NBP1-18889]

Western Blot: ABCF1 Antibody [NBP1-18889]

Western Blot: ABCF1 Antibody [NBP1-18889] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-18889 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABC50 was also immunoprecipitated by NBP1-18888, which recognizes an upstream epitope.
Immunohistochemistry: ABCF1 Antibody [NBP1-18889]

Immunohistochemistry: ABCF1 Antibody [NBP1-18889]

Immunohistochemistry: ABCF1 Antibody [NBP1-18889] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABC50 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .
Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-18889]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-18889]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-18889] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-ABC50 NBP1-18889 used at a dilution of 1:250.

Applications for ABCF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:2000-1:10000
Application Notes
It is not recommended for Immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ABCF1

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq]

Alternate Names

ABC27, ABC50TNFalpha-inducible ATP-binding protein, ATP-binding cassette 50, ATP-binding cassette 50 (TNF-alpha stimulated), ATP-binding cassette sub-family F member 1, ATP-binding cassette, sub-family F (GCN20), member 1, EC 2.7.7.6, EC 3.6.3.17, EST123147, TNF-alpha-stimulated ABC protein

Entrez Gene IDs

23 (Human)

Gene Symbol

ABCF1

UniProt

NP_001020262.1 (Human)

Additional ABCF1 Products

Product Documents for ABCF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ABCF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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