Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B5E10

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ABCG5 Antibodies »

Product Specifications

Immunogen

Partial recombinant human ABCG5 (between residues 300-500) expressed in E. coli [UniProt Q9H222].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ABCG5 Antibody (1B5E10) - Azide and BSA Free

Western Blot: ABCG5 Antibody (1B5E10)Azide and BSA Free [NBP2-80559]

Western Blot: ABCG5 Antibody (1B5E10)Azide and BSA Free [NBP2-80559]

Western Blot: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - Western blot analysis of ABCG5 in human ABCG5 recombinant protein. (Expected MW is 32.7 kDa) Image from the standard format of this antibody.
Immunohistochemistry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559]

Immunohistochemistry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559]

Immunohistochemistry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining. Image from the standard format of this antibody.
Flow Cytometry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559]

Flow Cytometry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559]

Flow Cytometry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - Flow cytometry analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.
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Applications for ABCG5 Antibody (1B5E10) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCG5

ABCG5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCG5 is a member of the White subfamily. It functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. ABCG5 is expressed in a tissue-specific manner in the liver, colon, and intestine. Mutations in this protein may contribute to sterol accumulation and atheroschlerosis, and they have been observed in patients with sitosterolemia (1).

Alternate Names

ATP-binding cassette sub-family G member 5, ATP-binding cassette, sub-family G (WHITE), member 5, ATP-binding cassette, subfamily G, member 5, sterolin 1, Sterolin-1, STSL

Gene Symbol

ABCG5

Additional ABCG5 Products

Product Documents for ABCG5 Antibody (1B5E10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ABCG5 Antibody (1B5E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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