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ABH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98658

Novus Biologicals, part of Bio-Techne
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NBP2-98658-100ul
NBP2-98658-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for ABH1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human ABH1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ABH1 Antibody

Western Blot: ABH1 Antibody [NBP2-98658]

Western Blot: ABH1 Antibody [NBP2-98658]

Western Blot: ABH1 Antibody [NBP2-98658] - Anti-ABH1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa
Immunohistochemistry-Paraffin: ABH1 Antibody [NBP2-98658]

Immunohistochemistry-Paraffin: ABH1 Antibody [NBP2-98658]

Immunohistochemistry-Paraffin: ABH1 Antibody [NBP2-98658] - Immunochemical staining of human ABH1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: ABH1 Antibody [NBP2-98658]

Immunoprecipitation: ABH1 Antibody [NBP2-98658]

Immunoprecipitation: ABH1 Antibody [NBP2-98658] - ABH1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-ABH1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ABH1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 43 kDa

Applications for ABH1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABH1

ABH1 encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.

Alternate Names

ABH1, ABHalkB, alkylation repair homolog (E. coli), ALKB, alkB, alkylation repair homolog 1 (E. coli), ALKBH, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, EC 1.14.11.-, EC 4.2.99.18, hABH

Gene Symbol

ALKBH1

Additional ABH1 Products

Product Documents for ABH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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