Abhd5 Antibody (1F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00051099-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Monkey

Cited:

Human, Mouse, Bovine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Abhd5 Antibodies »

Product Specifications

Immunogen

ABHD5 (AAH21958, 1 a.a. ~ 349 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD

Reactivity Notes

Mouse reactivity reported in multiple pieces of scientific literature

Specificity

ABHD5 - abhydrolase domain containing 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Abhd5 Antibody (1F3)

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01]

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01]

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 expression in HeLa, mouse and bovine tissues. Primary: 1:500 dilution. This image was submitted via customer Review.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in Jurkat(Cat # L017V1 ).
View 5 more images

Applications for Abhd5 Antibody (1F3)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell, tissue and transfected lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Knock Out Validation was reported in scientific literature (PMID: 27559856). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25894224).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using H00051099-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Abhd5

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Alternate Names

abhydrolase domain containing 5, Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5, CDS, CGI58, EC 2.3.1.51, IECN2, Lipid droplet-binding protein CGI-58, MGC8731, NCIE2CGI-58

Entrez Gene IDs

51099 (Human)

Gene Symbol

ABHD5

OMIM

275630 (Human)

UniProt

AAH21958 (Human)

Additional Abhd5 Products

Product Documents for Abhd5 Antibody (1F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Abhd5 Antibody (1F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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