Cytoplasm. Colocalizatd with PLIN and ADRP on the surface of lipid droplets. The localization is dependant upon the metabolic status of the adipocytes and the activity of PKA.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Abhd5 Antibody - BSA Free

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 Antibody [NB110-41576] - CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license.

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 Antibody [NB110-41576] - Abhd5 overexpression lysates (NBL1-07199).

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ATGL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blots are inset above each figure panel. GPAT, glycerol-3-phosphate acyltransferase; DGAT, diacylglycerol acyltransferase; ATGL, adipose triacylglycerol lipase; CGI-58, comparative gene identification 58; HSL, hormone sensitive lipase; AU, arbitrary units. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.

Simple Western: Abhd5 AntibodyBSA Free [NB110-41576]

Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Protein content of important players involved in lipid droplet dynamics in Caucasian and South Asian subjects before and after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 and (e) PLIN5 protein content. Data are presented as mean ± SEM and were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

The turnover of DAG and TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) and ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - A. ATGL (n = 8) & CGI-58 (n = 7) protein content in the three skeletal muscles (SOL, RG, & WG & heart).Ponceau stain for total protein was used as a loading control. Data are reported as mean±SE & bars with the same letter are not significantly different within the skeletal muscles (p<0.05). Insets: representative blots: lane 1, WG (white gastrocnemius); lane 2, RG (red gastrocnemius); lane 3, SOL (soleus). B. ATGL & CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - The turnover of DAG & TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) & ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0014503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the right ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Protein content of important players involved in lipid droplet dynamics in Caucasian & South Asian subjects before & after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 & (e) PLIN5 protein content. Data are presented as mean ± SEM & were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep42393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the left ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), G0/G1 switch gene 2 (G0S2), hormone sensitive lipase (HSL). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for Abhd5 Antibody - BSA Free

Application

Recommended Usage

Simple Western

1:1000

Western Blot

1:500

Application Notes

A band is seen at ~43 kDa in Western Blot. In ICC/IF cytoplasmic staining was observed in HeLa cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Abhd5

ABHD5 (abhydrolase domain containing protein 5; or lipid droplet-binding protein CGI-58) belongs to peptidase S33 family and it is a ubiquitously expressed enzyme which is critical to triacylglycerol (TAG) metabolism. Localized in the cytoplasm and lipid droplets, ABHD5 colocalized with PLIN/ADRP on lipid droplets surfaces. It is widely expressed in various tissues including lymphocytes, liver, skeletal muscle and brain. Biochemically, ABHD5 is a lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis and regulate the cellular storage of TAG through activation of phospholipase PNPLA2. ABHD5 mutations have been linked to neutral lipid storage disorder called Chanarin-Dorfman syndrome which is characterized by ichthyosis, hepatic steatosis, and excessive storage of TAG in multiple cells and tissues. ABHD5 lacks TAG hydrolase activity, but increases the lipase activity of ATGL through a poorly understood mechanism. In vitro and in vivo studies established that modulating expression of ABHD5 significantly alters lipolysis and levels of TAG, and endogenous ABHD5 expression are limiting in murine adipose tissue.

Alternate Names

abhydrolase domain containing 5, Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5, CDS, CGI58, EC 2.3.1.51, IECN2, Lipid droplet-binding protein CGI-58, MGC8731, NCIE2CGI-58

Gene Symbol

ABHD5

Additional Abhd5 Products

Product Documents for Abhd5 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for Abhd5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Abhd5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-41576

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Abhd5 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for Abhd5 Antibody - BSA Free

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 AntibodyBSA Free [NB110-41576]

Simple Western: Abhd5 AntibodyBSA Free [NB110-41576]

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] -

Applications for Abhd5 Antibody - BSA Free

Simple Western

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Abhd5

Alternate Names

Gene Symbol

Additional Abhd5 Products

Product Documents for Abhd5 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Abhd5 Antibody - BSA Free