ACAA1 Antibody (CL2662) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42381
![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0002.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
![Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Western-Blot-NBP2-42381-img0001.jpg "Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL2662
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P09110, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected to cross react based on sequence identity: Mouse (88%), Rat (87%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for ACAA1 Antibody (CL2662) - BSA Free
Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381]
Western Blot: ACAA1 Antibody (CL2662) [NBP2-42381] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251![Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunocytochemistry-Immunofluorescence-NBP2-42381-img0007.jpg "Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381]")
Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381]
Immunocytochemistry/Immunofluorescence: ACAA1 Antibody (CL2662) [NBP2-42381] - Staining of CACO-2 cells using the Anti-ACAA1 monoclonal antibody, showing specific staining in peroxisomes in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0006.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]
Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0003.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0004.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
![Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]](https://resources.bio-techne.com/images/products/ACAA1-Antibody-CL2662-Immunohistochemistry-NBP2-42381-img0005.jpg "Immunohistochemistry: ACAA1 Antibody (CL2662) [NBP2-42381]")
Applications for ACAA1 Antibody (CL2662) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ACAA1
Alternate Names
Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 1, EC 2.3.1, peroxisomal
Gene Symbol
ACAA1
UniProt
Additional ACAA1 Products
Product Documents for ACAA1 Antibody (CL2662) - BSA Free
Product Specific Notices for ACAA1 Antibody (CL2662) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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