Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C5

Format

BSA Free

Concentration

1 mg/ml

View all ACAT1 Antibodies »

Product Specifications

Immunogen

Recombinant human ACAT1 (34-427aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ACAT1 Antibody (2C5) - BSA Free

Western Blot: ACAT1 Antibody (2C5)BSA Free [NBP2-22565]

Western Blot: ACAT1 Antibody (2C5)BSA Free [NBP2-22565]

Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.
Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody (2C5) - BSA Free [NBP2-22565]

Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody (2C5) - BSA Free [NBP2-22565]

Immunocytochemistry/Immunofluorescence: ACAT1 Antibody (15E5) [NBP2-22565] - Analysis of ACAT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: ACAT1 Antibody (2C5)BSA Free [NBP2-22565]

Western Blot: ACAT1 Antibody (2C5)BSA Free [NBP2-22565]

Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for ACAT1 Antibody (2C5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAT1

ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction. Defects in ACAT1 are a cause of 3-ketothiolase deficiency. 3-ketothiolase deficiency is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded.

Long Name

Acetyl-CoA Acetyltransferase 1

Alternate Names

THIL

Gene Symbol

ACAT1

UniProt

NP_000010 (Human)

Additional ACAT1 Products

Product Documents for ACAT1 Antibody (2C5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACAT1 Antibody (2C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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