ACE-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76614

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ACE-2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the center of human ACE-2. The immunogen is located within amino acids 180 - 230 of ACE-2. Amino Acid Squence: RANHYEDYGDYWRGDYE

Specificity

Anti-ACE2 has no cross response to ACE1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACE-2 Antibody - BSA Free

Western Blot: ACE-2 AntibodyBSA Free [NBP1-76614]

Western Blot: ACE-2 AntibodyBSA Free [NBP1-76614]

Western Blot: ACE-2 Antibody [NBP1-76614] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), ACE2, (2 ug/mL), ACE2, (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76614]

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76614]

Immunocytochemistry/Immunofluorescence: ACE-2 Antibody [NBP1-76614] - Analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with 20 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry-Paraffin: ACE-2 Antibody - BSA Free [NBP1-76614]

Immunohistochemistry-Paraffin: ACE-2 Antibody - BSA Free [NBP1-76614]

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-76614] - Analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Applications for ACE-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20ug/ml

Immunohistochemistry

2ug/ml

Immunohistochemistry-Paraffin

2ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACE-2

Angiotensin I Converting Enzyme 2 (ACE2), also known as ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family (theoretical molecular weight 92 kDa). The predicted mouse ACE2 protein sequence consists of 798 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. Mouse ACE2 is 40% homologous in amino acid identity to the N- and C-terminal domains of mouse somatic ACE. Enzymatically, ACE2 converts the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts Ang I to Ang II.

Genetic data from Drosophila, mice and rats show that the ACE2 protein is an essential regulator of heart function in vivo. ACE2 mRNA is found at high levels in testis, kidney and heart with moderate levels in colon, small intestine, and ovary. The ACE2 protein contributes to organ function through the renin-angiotensin system, playing a central role in vascular, renal, and myocardial physiology.

Virology research has demonstrated that the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein binds to its functional receptor, ACE2 (1). Vimentin is a type III intermediate filament protein expressed in mesenchymal cells that helps comprise the cytoskeleton in all animal cells. Studies have demonstrated that vimentin allows cell binding that allows the uptake of SARS-CoV spike protein into a host (2). This direct interaction of SARS-CoV with vimentin has been identified as an entry mechanism for the virus into a host, mediated by the SARS-CoV receptor ACE2 (1,2). It has been shown that ACE2 is the SARS-CoV-2 receptor required for cell entry and plays a physiological role in the replication of SARS-CoV in an infected host (1). Studies using human ACE2 antibody demonstrated a blockade of SARS-CoV-2 and ACE2 interaction, indicating an important physiological component of viral transmission and potential anti-viral therapeutic strategies (3).

References

1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A., . . . Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454. doi:10.1038/nature02145

2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci. doi: 10.1186/s12929-016-0234-7

3. Hoffmann, M., Kleine-Weber, H., Schroeder, S., Kruger, N., Herrler, T., Erichsen, S., . . . Pohlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. doi:10.1016/j.cell.2020.02.052

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Gene Symbol

ACE2

UniProt

NP_068576 (Human)

Additional ACE-2 Products

Product Documents for ACE-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ACE-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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