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Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05146

Novus Biologicals, part of Bio-Techne
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NBP1-05146-0.025mg
NBP1-05146-0.1mg

Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E11

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme.

Epitope

C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1).

Reactivity Notes

Human and Bovine.

Specificity

Specific for brain AChE and does not recognize AChE from erythrocytes. The can thus distinguish between mammalian brain AChE and erythrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.

Marker

Early Neuronal Development Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free [NBP1-05146]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free [NBP1-05146]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free [NBP1-05146] - Human placenta stained with NBP1-05146

Applications for Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

Application
Recommended Usage

Western Blot

1:75

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Acetylcholinesterase/ACHE

Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetylcholinesterase/ACHE Antibody (4E11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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