Aconitase 2 Antibody (0P3H3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16426
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0P3H3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Aconitase 2 (Q99798). MAPYSLLVTRLQKALGVRQYHVASVLCQRAKVAMSHFEPNEYIHYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aconitase 2 Antibody (0P3H3)
Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426]
Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (0P3H3) [NBP3-16426]
Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunofluorescence analysis of NIH-3T3 cells using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Aconitase 2 Antibody (0P3H3) [NBP3-16426]
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry of paraffin-embedded mouse kidney using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Aconitase 2 Antibody (0P3H3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aconitase 2
Alternate Names
Aconitase, aconitase 2, mitochondrial, aconitate hydratase, mitochondrial, ACONM, Citrate hydro-lyase, EC 4.2.1, EC 4.2.1.3, MGC20605, MGC33908
Gene Symbol
ACO2
Additional Aconitase 2 Products
Product Documents for Aconitase 2 Antibody (0P3H3)
Product Specific Notices for Aconitase 2 Antibody (0P3H3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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