Aconitase 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90264
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aconitase 2 Antibody
Immunohistochemistry: Aconitase 2 Antibody [NBP1-90264]
Immunohistochemistry: Aconitase 2 Antibody [NBP1-90264] - STED super-resolution microscopy of mitochondria in the rectal Muscularis externa demonstrates high structural preservation of the stored paraffin-embedded tissue. STED recordings were performed on 2 um thick dewaxed sections cut along the longitudinal axis of the rectum. STED images of tissue sections decorated with antisera against aconitase. In each panel the confocal (left) and the corresponding STED image (right) is displayed. Magnification of the STED image as indicated by a dashed square. Scale bar: 200 nm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0101563), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody [NBP1-90264]
Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-90264] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.Applications for Aconitase 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aconitase 2
Alternate Names
Aconitase, aconitase 2, mitochondrial, aconitate hydratase, mitochondrial, ACONM, Citrate hydro-lyase, EC 4.2.1, EC 4.2.1.3, MGC20605, MGC33908
Gene Symbol
ACO2
UniProt
Additional Aconitase 2 Products
Product Documents for Aconitase 2 Antibody
Product Specific Notices for Aconitase 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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