ACSF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47558

Novus Biologicals, part of Bio-Techne
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NBP2-47558
NBP2-47558-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACSF2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSKDMIIRGGEN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACSF2 Antibody

Immunohistochemistry-Paraffin: ACSF2 Antibody [NBP2-47558]

Immunohistochemistry-Paraffin: ACSF2 Antibody [NBP2-47558]

Immunohistochemistry-Paraffin: ACSF2 Antibody [NBP2-47558] - Staining in human kidney and smooth muscle tissues using anti-ACSF2 antibody. Corresponding ACSF2 RNA-seq data are presented for the same tissues.
Western Blot: ACSF2 Antibody [NBP2-47558]

Western Blot: ACSF2 Antibody [NBP2-47558]

Western Blot: ACSF2 Antibody [NBP2-47558] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: ACSF2 Antibody [NBP2-47558]

Immunocytochemistry/ Immunofluorescence: ACSF2 Antibody [NBP2-47558]

Immunocytochemistry/Immunofluorescence: ACSF2 Antibody [NBP2-47558] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
View 2 more images

Applications for ACSF2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western internal validation: Separated by size; antibody dilution of 1:20; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSF2

Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA.Has some preference toward medium-chain substrates. Plays a role in adipodyte differentiation

Alternate Names

ACSMW, acyl-CoA synthetase family member 2, AVYV493, EC 6.2.1, EC 6.2.1.-, EC 6.2.1.26, mitochondrial, PPARG binding, long chain fatty acid acyl Co-A ligase like

Gene Symbol

ACSF2

Additional ACSF2 Products

Product Documents for ACSF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACSF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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