ACSL1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-99585

Novus Biologicals, part of Bio-Techne
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NB110-99585
NB110-99585SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ACSL1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of the human ACSL1 protein (within residues 1-100). [Swiss-Prot# P33121]

Localization

Single-pass type III membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACSL1 Antibody - BSA Free

Western Blot: ACSL1 AntibodyBSA Free [NB110-99585]

Western Blot: ACSL1 AntibodyBSA Free [NB110-99585]

Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody - BSA Free [NB110-99585]

Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody - BSA Free [NB110-99585]

Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NB110-99585] - ACSL1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: ACSL1 AntibodyBSA Free [NB110-99585]

Simple Western: ACSL1 AntibodyBSA Free [NB110-99585]

Simple Western: ACSL1 Antibody [NB110-99585] - Simple Western lane view shows a specific band for ACSL1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for ACSL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25 - 1:100

Simple Western

1:100

Western Blot

1 ug/ml
Application Notes
A band is seen approx. 78 kDa. In ICC/IF membrane staining was observed in HepG2 cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSL1

ACSL1 (long-chain-fatty-acid--CoA ligase 1), belongs to ATP-dependent AMP-binding enzyme family and is one of the 5 isoforms (ACSL - 1, -3, -4, -5 and -6) of long chain acyl CoA synthetases (ACSLs), the enzymes which plays a crucial role in regulating fatty acid metabolism by converting free fatty acids (FFAs) into fatty acyl CoA derivatives via a process called fatty acid activation, a modification required for any FFA to undergo further metabolism. ACSL1 is a single-pass type III membrane protein localized in mitochondrion outer membrane and membranes of peroxisomes, microsomes, ER of energy-metabolizing tissues such as fat, liver, and skeletal muscles. ACSL1 is a central enzyme in the intermediary metabolism due to its fundamental role in activation of long-chain fatty acids through the incorporation of CoA. These bioactive fatty acid metabolites are involved in many cellular biochemical processes, including protein transport, enzyme activation, and transcriptional control. Additionally, fatty acyl-CoAs are used as substrates in fatty acid beta-oxidation and the phospholipid biosynthesis pathways.

Alternate Names

ACS1LACS 2, Acyl-CoA synthetase 1, acyl-CoA synthetase long-chain family member 1, EC 6.2.1, FACL1EC 6.2.1.3, fatty-acid-Coenzyme A ligase, long-chain 1, LACS 1, LACSlong-chain 2, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, long-chain fatty-acid-coenzyme A ligase 1, long-chain-fatty-acid--CoA ligase 1, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, paltimoyl-CoA ligase 1

Gene Symbol

ACSL1

Additional ACSL1 Products

Product Documents for ACSL1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACSL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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