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ACTH Antibody (CLIP/7197R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23601

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-23601-100ug
NBP3-23601-20ug

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # CLIP/7197R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

N-terminal fragment of human ACTH conjugated to KLH

Reactivity Notes

Expected to show a broad species reactivity.

Localization

Cytoplasm. Secreted.

Specificity

This monoclonal antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Marker

Pituitary Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for ACTH Antibody (CLIP/7197R)

ACTH Antibody (CLIP/7197R)

Immunohistochemistry-Paraffin: ACTH Antibody (CLIP/7197R) [NBP3-23601] -

Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH antibody (CLIP/7197R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for ACTH Antibody (CLIP/7197R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ACTH

ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.

Long Name

Adrenocorticotropic Hormone

Alternate Names

Adrenocorticotropin, Corticotropin

Gene Symbol

POMC

Additional ACTH Products

Product Documents for ACTH Antibody (CLIP/7197R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACTH Antibody (CLIP/7197R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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