ACTH Antibody (rCLIP/1407)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07626
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rCLIP/1407
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to (aa 162-176) of human ACTH (NGAEDESAEAFPLEF) (Uniprot: P01189 )
Epitope
aa1-24
Reactivity Notes
Expected to show broad species reactivity.
Localization
Cytoplasmic
Specificity
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This monoclonal antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Marker
Pituitary Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08617)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ACTH Antibody (rCLIP/1407)
Immunohistochemistry-Paraffin: ACTH Antibody (rCLIP/1407) [NBP3-07626]
Immunohistochemistry-Paraffin: ACTH Antibody (rCLIP/1407) [NBP3-07626] - Formalin-fixed, paraffin-embedded human pituitary stained with ACTH Mouse Recombinant Monoclonal Antibody (rCLIP/1407).Immunohistochemistry-Paraffin: ACTH Antibody (rCLIP/1407) [NBP3-07626]
Immunohistochemistry-Paraffin: ACTH Antibody (rCLIP/1407) [NBP3-07626] - Formalin-fixed, paraffin-embedded human pituitary stained with ACTH Mouse Recombinant Monoclonal Antibody (rCLIP/1407).Applications for ACTH Antibody (rCLIP/1407)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ACTH
Long Name
Adrenocorticotropic Hormone
Alternate Names
Adrenocorticotropin, Corticotropin
Gene Symbol
POMC
UniProt
Additional ACTH Products
Product Documents for ACTH Antibody (rCLIP/1407)
Product Specific Notices for ACTH Antibody (rCLIP/1407)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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