Actin Gamma 1 Antibody (NH3)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64792

Novus Biologicals, part of Bio-Techne
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NB100-64792SS
NB100-64792
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # NH3

Concentration

1.0 mg/ml

View all Actin Gamma 1 Antibodies »

Product Specifications

Immunogen

Human monocytes and U937 cell line

Reactivity Notes

Predicted cross-reactivities: Rat, Rabbit, Mouse

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Actin Gamma 1 Antibody (NH3)

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.
Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse at 1:200. Nuclei stained with DAPI. IHC-P image submitted by a verified customer review.

Applications for Actin Gamma 1 Antibody (NH3)

Application
Recommended Usage

ELISA

1:10

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Western Blot

1:100 - 1:500
Application Notes
Actin Gamma 1 antibody validated for ICC/IF and IHC-P from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 4 using NB100-64792 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Actin Gamma 1

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Entrez Gene IDs

71 (Human)

Gene Symbol

ACTG1

UniProt

P63261 (Human)

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody (NH3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Actin Gamma 1 Antibody (NH3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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