Actin (Muscle Specific) Antibody (HHF35)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34230

Novus Biologicals, part of Bio-Techne
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NBP2-34230-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HHF35

Concentration

0.2 mg/ml

View all Actin (Muscle Specific) Antibodies »

Product Specifications

Immunogen

SDS extract of human myocardium.

Localization

Cytoplasmic

Specificity

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Marker

Muscle Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34634)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Actin (Muscle Specific) Antibody (HHF35)

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230]

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230]

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)
Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230]

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230]

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).
View 3 more images

Applications for Actin (Muscle Specific) Antibody (HHF35)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Simple Western

10 ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Actin (Muscle Specific)

Alternate Names

ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3, 1a4 sma, 1a4 smooth muscle actin, alpha sma 1a4, anti-alpha SMA, sma 1a4

Gene Symbol

ACTA1

UniProt

P68032, P68133 (Human), P62736 (Human)

Additional Actin (Muscle Specific) Products

Product Documents for Actin (Muscle Specific) Antibody (HHF35)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Actin (Muscle Specific) Antibody (HHF35)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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