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Actin (Muscle Specific) Antibody (rMSA/953) [Janelia Fluor® 635]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14096JF635

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat, Rabbit

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Janelia Fluor 635

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rMSA/953

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Actin (Muscle Specific) Antibody (rMSA/953) [Janelia Fluor® 635]

Immunogen

Recombinant protein corresponding to human muscle actin

Localization

Cytoplasm

Specificity

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Marker

Muscle Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Actin (Muscle Specific) Antibody (rMSA/953) [Janelia Fluor® 635]

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Actin (Muscle Specific)

Alternate Names

ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3

Gene Symbol

ACTA1

Additional Actin (Muscle Specific) Products

Product Documents for Actin (Muscle Specific) Antibody (rMSA/953) [Janelia Fluor® 635]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Actin (Muscle Specific) Antibody (rMSA/953) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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