Activin A Antibody

Western Blot: Activin A Antibody [NBP1-30928]

Western Blot: Activin A Antibody [NBP1-30928] - Western blot analyses of YY1 and inhibin betaA in MDA-231 cells co-transfected with miR-218 mimic and YY1-overexpressing plasmid, or the corresponding controls at 72 h. Image collected and cropped by CiteAb from the following publication (breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-1059-y), licensed under a CC-BY license.

Western Blot: Activin A Antibody [NBP1-30928] -

Western Blot: Activin A Antibody [NBP1-30928] - miR-218 directly targets INHBB & YY1, & increases INHBA expression. a Relative RNA level of INHBB & INHBA normalized to 18S in MDA-231 transfected with miR-218 or control miRNA mimic at 24 h & 72 h, respectively. ***P < 0.001. b Western blot analyses of inhibin betaB & inhibin betaA in mimic-transfected MDA-231 cells at 48 h. c Relative RNA level of INHBB & INHBA normalized to 18S in MDA-231-bone transfected with anti-miR-218 or control antagomiR at 24 h & 48 h, respectively.. **P < 0.01; ***P < 0.001. d Sequence alignment of miR-218 & its predicted targets INHBB & YY1. e psiCheck2 reporter plasmids & miR-218 mimic or its corresponding control were transfected into indicated cell lines. Renilla & firefly luciferase activity was measured at 48 h. *P < 0.05; n.s. not significant. f Relative RNA level of YY1 normalized to 18S in mimic-transfected MDA-231 cells at 24 h. ***P < 0.001. g Western blot analyses of YY1 & inhibin betaA in MDA-231 cells co-transfected with miR-218 mimic & YY1-overexpressing plasmid, or the corresponding controls at 72 h. h psiCheck2 reporter plasmids & miR-218 mimic or its corresponding control vector were transfected into MDA-231 cells. Renilla & firefly luciferase activity was measured at 48 h. **P < 0.01; n.s. not significant Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30348200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Activin A Antibody [NBP1-30928] -

Western Blot: Activin A Antibody [NBP1-30928] - miR-218-regulated inhibin betaA affects SMAD2/3 signaling in a cell-dependent manner. a & b Western blot analyses of phospho-SMAD2/3 in MDA-231 (a) or MC3T3 (b) that was serum-starved overnight & treated with EV-depleted CM collected from indicated cells for 30 min or 2 h. CM producing cell lines were transfected, PBS washed 48 h after transfection & then incubated with serum-free medium overnight before CM collection & EV depletion by ultracentrifugation. c Western blot analyses of inhibin betaA, inhibin betaB, & inhibin alpha in CM from indicated cell lines that was concentrated with medium concentrator columns. Arrows indicate the position of antigen. Control mimic-transfected MDA-231 whole cell lysate (WCL) was used as positive control for inhibin betaA & inhibin betaB. d Western blot analyses of inhibin betaA monomer or dimer (activin A) in CM from indicated cell lines under non-reducing condition & without boiling the samples. Bottom & top arrows indicate inhibin betaA monomer & dimer, respectively. e Western blot analyses of inhibin alpha in MC3T3 CM concentrated from 20 ml CM. WCL of MCF10A was used as a positive control. f & g Western blot analyses of phospho-SMAD2/3 in MDA-231 (f) or MC3T3 (g) that was serum-starved overnight & treated with CM collected from indicated cells for 30 min or 2 h. For MDA-231 cells (f), recombinant inhibin alpha protein was added to CM as indicated. For MC3T3 cells (g), anti-inhibin alpha antibody was added to CM as indicated Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30348200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Activin A Antibody [NBP1-30928] -

Western Blot: Activin A Antibody [NBP1-30928] - Non-transfected (–) and transfected (+) PC-3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.