Activin RIB/ALK-4 Antibody (JE38-48)

Immunohistochemistry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998]

Immunohistochemistry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31998) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998]

Immunocytochemistry/ Immunofluorescence: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Immunocytochemistry analysis of U-87 MG cells labeling Activin RIB/ALK-4 with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998]

Flow Cytometry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Flow cytometric analysis of U-87 MG cells labeling Activin RIB/ALK-4.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31998, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).