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ACTL6B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18635

Novus Biologicals, part of Bio-Techne
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NBP3-18635-100ul
NBP3-18635-25ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against human ACTL6B (actin-like 6B), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for ACTL6B Antibody

Western Blot: ACTL6B Antibody [NBP3-18635]

Western Blot: ACTL6B Antibody [NBP3-18635]

Western Blot: ACTL6B Antibody [NBP3-18635] - Figure 2. Western blot analysis using the antibody directed against ACTL6B Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ACTL6B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
ELISA: ACTL6B Antibody [NBP3-18635]

ELISA: ACTL6B Antibody [NBP3-18635]

ELISA: ACTL6B Antibody [NBP3-18635] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against ACTL6B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000

Applications for ACTL6B Antibody

Application
Recommended Usage

ELISA

1:500-1:1000

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: ACTL6B

The protein encoded by the ACTL6B gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. (provided by RefSeq)

Alternate Names

53 kDa BRG1-associated factor B, actin-like 6, actin-like 6B, Actin-related protein Baf53b, ACTL6, ArpNalpha, BAF53Bactin-like protein 6B, BRG1-associated factor 53B, hArpN alpha

Gene Symbol

ACTL6B

UniProt

Additional ACTL6B Products

Product Documents for ACTL6B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACTL6B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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