ACTR1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1424

Novus Biologicals, part of Bio-Techne
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NB100-1424
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ACTR1B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YEEDGSRAIHRKT corresponding to C-Terminus according to NP_005726.1.

Localization

Cytoplasmic

Specificity

This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACTR1B Antibody

Western Blot: ACTR1B Antibody [NB100-1424]

Western Blot: ACTR1B Antibody [NB100-1424]

Western Blot: ACTR1B Antibody [NB100-1424] - (1ug/ml) Analysis human heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry: ACTR1B Antibody [NB100-1424]

Immunohistochemistry: ACTR1B Antibody [NB100-1424]

Immunohistochemistry: ACTR1B Antibody [NB100-1424] - (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ACTR1B Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human heart and brain lysates (predicted MW of 43 kDa band according to NP_005726). IHC-P: Human uterus shows fibrillar staining of cytoplasm in endometrial cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTR1B

In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90% amino acid identity and 96% amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be ivolved in dynein-driven vesicle movement.

Alternate Names

Actin-related protein 1B, ARP1 (actin-related protein 1, yeast) homolog B (centractin beta), ARP1 actin-related protein 1 homolog B, centractin beta (yeast), ARP1BPC3, centractin beta, CTRN2beta-centractin

Entrez Gene IDs

10120 (Human); 226977 (Mouse)

Gene Symbol

ACTR1B

UniProt

P42025 (Human)

Additional ACTR1B Products

Product Documents for ACTR1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACTR1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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